After that, these validated primers can be synthesized for use in the amplification of concerned gene/DNA fragment. Primer 3 (on line) Gene Runner 1.Sequence Insertion Oligo 6 Manual 1. such as gene discovery, microRNA, methylation, SNPs, and primer design. Gene Runner Primer Design Primer Designing 1. Primers were designed ( Table 3 ) using the software GENE RUNNER Version 3.4.0.0 (Hasting. Thereafter, the primers designed in silico can be validated in the wet lab. Gene Runner Cracked 2022 Latest Version is a tool designed by Biomatters LTD. The SMPD1 gene is located on chromosome 11 at p15.1p15.4. The in silico validation can be carried out using BLAST tool and Gene Runner software, which check their efficiency and specificity. For designing the primer, DNA template sequence is required that can be taken from any of the available sequence databases, e.g., RefSeq database. Here we described designing of primers using web-based tools like "Primer3" and "Web Primer". There are various programs available for PCR primer design. In silico method helps in designing primers. Selective amplification of nucleic acid molecules initially present in minute quantities provides a powerful tool for analyzing nucleic acids. Polymerase chain reaction (PCR) is an enzymatic reaction whose efficiency and sensitivity largely depend on the efficiency of the primers that are used for the amplification of a concerned gene/DNA fragment.
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